Comparison and characterization of immunoglobulin G subclasses among primate species.

Detection, Cloning, Molecular Characterization and Phylogenic Analyses of a New Primate T-Cell Lymphotropic Virus Type I in Olive Baboon

Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins

Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of ma...

Molecular and functional characterization of cynomolgus monkey IgG subclasses.

Studies for vaccine and human therapeutic Ab development in cynomolgus monkeys (cynos) are influenced by immune responses, with Ab responses playing a significant role in efficacy and immunogenicity. Understanding the nature of cyno humoral immune responses and characterizing the predominant cyno IgG types produced and the Fc-FcγR interactions could provide insight into the immunomodulatory eff...

Specificity of Fc receptors induced by herpes simplex virus type 1: comparison of immunoglobulin G from different animal species.

Cells infected with herpes simplex virus type 1 (HSV-1) express a cell surface receptor able to bind the Fc portion of immunoglobulin G (IgG). Of the four human IgG subclasses, the HSV-1 Fc receptor, like staphylococcal protein A, binds to all except IgG3. In this paper, we describe the binding of a number of animal IgG and IgG subclass molecules to HSV-1-infected cells and compare this binding...

Different Somatic Hypermutation Levels among Antibody Subclasses Disclosed by a New Next-Generation Sequencing-Based Antibody Repertoire Analysis

A diverse antibody repertoire is primarily generated by the rearrangement of V, D, and J genes and subsequent somatic hypermutation (SHM). Class-switch recombination (CSR) produces various isotypes and subclasses with different functional properties. Although antibody isotypes and subclasses are considered to be produced by both direct and sequential CSR, it is still not fully understood how SH...